Efficient co-stabilization of arsenic and cadmium in farmland soil by schwertmannite under long-term flooding-drying condition.

Simultaneously stabilizing of arsenic (As) and cadmium (Cd) in co-contaminated soil presents substantial challenges due to their contrasting chemical properties. Schwertmannite (Sch) is recognized as a potent adsorbent for As pollution, with alkali modification showing promising results in the simultaneous immobilization of both As and Cd. This study systematically investigated the long-term stabilization efficacy of alkali-modified Sch in Cd-As co-contaminated farmland soil over a 200-day flooding-drying period. The results revealed that As showed significant mobility in flooded conditions, whereas Cd exhibited increased soil availability under drying phases. The addition of Sch did not affect the trends in soil pH and Eh fluctuations; nonetheless, it led to an augmentation in the levels of amorphous iron oxides and SO42- concentration in soil pore water. At a dosage of 0.5% Sch, there was a notable decrease in the mobility and soil availability of As and Cd under both flooding (34.5% and 53.6% at Day 50) and drying conditions (27.0% and 29.4% at Day 130), primarily promoting the transformation of labile metal(loid) fraction into amorphous iron oxide-bound forms. Throughout the flooding-drying treatment period, Sch maintained stable mineral morphology and mineralogical phase, highlighting its long-term stabilization effect. The findings of this study emphasize the promising application of Sch-based soil remediation agents in mitigating the challenges arising from As-Cd co-contamination. Further research is warranted to explore their application in real farmland settings and their impact on the uptake of toxic metal(loid)s by plants.

Aeration pre-treatment role in improving the performance of bio-conditioning dewatering of food waste anaerobic digestate.

Bio-conditioning dewatering followed by activated sludge process (BDAS) is a promising technology for purifying food waste anaerobic digestate (FWAD). However, the bio-conditioning dewatering efficiency is often affected by FWAD properties and ambient temperature. Here, we firstly reported that aeration pre-treatment of FWAD played an important role in improving the bio-conditioning dewatering performance of FWAD. The study found that the accumulated carbonate (CO32-) in FWAD severely affected the flocculation of Fe-containing flocculant formed in microbial fermentation liquor due to the competitive consumption of the flocculant by CO32-. The capillary suction time (CST) and specific resistance to filtration (SRF) of the bio-conditioned FWAD increased from initial 77.8 s and 2.0 × 1012 m/kg to 122.7 s and 3.4 × 1012 m/kg, respectively, within 1 day of aeration. Prolonged aeration pre-treatment of FWAD could reduce its CO32- concentration and total alkalinity. Additionally, the aeration pre-treatment simultaneously decreased the proportion of macromolecular organic matter that hindered dewatering and the content of total solids (TS) and hydrophilic protein-like substances in FWAD. After 20 days of aeration followed by bio-conditioning, the CST and SRF reduced to final 36.5 s and 2.3 × 1011 m/kg, respectively, indicating a substantial improvement in dewatering performance. Successive forced aeration combined with the addition of CaCl2 to eliminate adverse factors mainly CO32- was a feasible and cost-effective strategy to realize bio-conditioning dewatering of FWAD in less than 2 days and a lower reagents dose of bio-conditioning, which was helpful in the engineering application of the novel BDAS process for FWAD purification.

A novel approach for purifying food waste anaerobic digestate through bio-conditioning dewatering followed by activated sludge process: A case study.

Although anaerobic digestion is the mainstream technology for treating food waste (FW), the high pollutant concentration in the resultant food waste anaerobic digestate (FWAD) often poses challenges for the subsequent biochemical treatment such as activated sludge process. In this study, taking a typical FW treatment plant as an example, we analyzed the reasons behind the difficulties in treating FWAD and tested a novel process called as bio-conditioning dewatering followed by activated sludge process (BDAS) to purify FWAD. Results showed that high concentrations of suspended solids (SS) (16439 ± 475 mg/L), chemical oxygen demand (COD) (24642 ± 1301 mg/L), and ammonium nitrogen (NH4+-N) (2641 ± 52 mg/L) were main factors affecting the purification efficiency of FWAD by the conventional activated sludge process. By implementing bio-conditioning dewatering for solid-liquid separation, near 100% of SS and total phosphorus (TP), 90% of COD, 38% of total nitrogen (TN), and 37% of NH4+-N in the digestate could be effectively removed or recovered, consequently generating the transparent filtrate with relatively low pollution load and dry sludge cake (<60% of moisture content). Furthermore, after ammonia stripping and biochemical treatment, the effluent met the relevant discharge standards regulated by China, with the concentrations of COD, TN, NH4+-N, and TP ranging from 151 to 405, 10-56, 0.9-31, and 0.4-0.8 mg/L, respectively. This proposed BDAS approach exhibited stable performance and low operating costs, offering a promising solution to purify FWAD in practical engineering and simultaneously realize resource recovery.

Imaging objects hidden inside the strongly scattering media based on bidirectional ghost imaging.

We demonstrate a novel, to the best of our knowledge, method for imaging objects hidden inside the strongly scattering media based on bidirectional ghost imaging (GI). In this method, GI is performed separately on both sides of the object, resulting in two GI results. Through an autocorrelation operation to the two GI results, the convolution between the autocorrelation of the object and the point spread function (PSF) of the strongly scattering media can be recovered. Therefore, the object can be recovered by obtaining the PSF of the strongly scattering media through noninvasive measurement or numerical calculation. Simulation and experimental results show that bidirectional ghost imaging (BGI) can reconstruct high-quality images, particularly when the thickness of the strongly scattering media greatly exceeds the scattering mean free path.

A method of constructing a dynamic chart depth model for coastal areas.

The depth is important for vessel navigation at sea. Currently, most vessels use electronic navigation charts to navigate at sea. In coastal areas, especially close to shallow water areas, the dynamic change of the water level is very important to safe navigation. Ships calculate the change of water level by using up-to-date tide tables, to obtain the dynamic water depth in the channels. However, the depth caused by the tide and non-tidal components may reach several meters in some seas, causing the dynamic depth below the safety depth, which can easily lead to grounding of vessels stranding accidents. The channel is regularly dredged to achieve navigational depth. Without regular dredging, the offshore non-channel area becomes the common area of ship grounding. The dynamic chart depth model studied in this article can provide real-time depth, which serves the ships navigation in the non-channel. The model incorporates the chart depth and the dynamic water levels on the same reference datum. The chart depth is from the electronic navigational chart depth. The dynamic water levels are constructed by the simulated tidal levels and continuous series of nontidal residual. We then designed a deviation correction method to reduce the discrepancy of the simulated tidal level with the actual water level, including datum offset correction and residual water level correction. Finally, by merging the revised dynamic water levels with the electronic navigational chart depth, we obtained the dynamic chart depth model of the study region.

Feasibility of Bio-Coagulation Dewatering Followed by Bio-Oxidation Process for Treating Swine Wastewater.

The unsatisfactory performance of the conventional swine wastewater treatment is drawing increasing attention due to the large amount of refractory chemical oxygen demand (COD), nitrogen, and phosphorus attached to the suspended solids (SS). In this study, for the first time, a novel process based on bio-coagulation dewatering followed by a bio-oxidation (BDBO) system was developed to treat swine wastewater containing high-strength SS, COD, TN, and TP. Firstly, after the bio-coagulation process, the removal efficiencies of SS, COD, NH3-N, and TP reached as high as 99.94%, 98.09%, 61.19%, and 99.92%, respectively. Secondly, the filtrate of the bio-coagulation dewatering process was introduced into the subsequent bio-oxidation process, in which the residual COD and NH3-N were further biodegraded in a sequence batch reactor. In addition, the dewatering performance of the concentrated swine slurry was substantially improved, with the specific resistance to filtration decreasing from 17.0 × 1012 to 0.3 × 1012 m/kg. Moreover, the concentrated swine slurry was pressed and filtered into a semi-dry cake after pilot-scale bio-coagulation dewatering treatment. Finally, the concentrations of COD and NH3-N in the effluent after the BDBO process, ranging between 150-170 mg/L and 75-90 mg/L, met the relevant discharge standard. Compared to traditional treatments, the BDBO system has excellent large-scale potential for improving the treatment efficiency, shortening the operation period, and reducing the processing costs, and is emerging as a cost-effective alternative for the treatment of wastewater containing high concentrations of SS, COD, TN, and TP.

Elucidating interactive effects of sulfidated nanoscale zero-valent iron and ammonia on anaerobic digestion of food waste.

In our previous study, anaerobic digestion of food waste could be effectively enhanced by adding sulfidated nanoscale zero-valent iron (S-nZVI) under high-strength ammonia concentrations. In this study, in order to further elucidate the specific interactive effects of S-nZVI and ammonia on anaerobic digestion of nitrogen-rich food waste, the methanogenic performance of anaerobic digestion systems respectively added with nanoscale zero-valent iron (nZVI) and S-nZVI were compared and monitored under different ammonia stress conditions. Both nZVI and S-nZVI could effectively stimulate the methanogenesis process among ammonia concentrations ranging from 0 to 3500 mg/L. However, the enhancing effects of S-nZVI and nZVI on anaerobic digestion of food waste were different, in which anaerobic digestion systems added with S-nZVI and nZVI performed best under 2500 mg/L of ammonia and 1500 mg/L of ammonia, respectively. Furthermore, the analysis of microbial communities suggested that ammonia stress enriched acetoclastic methanogens, while adding nZVI and S-nZVI into anaerobic digestions stimulated the process of hydrogenotrophic methanogenesis. Moreover, S-nZVI performed better in promoting the evolution of DIET-related microorganisms than nZVI, resulting in enhanced methane production under high ammonia-stressed conditions. This work provided fundamental knowledge about the interactive effects of S-nZVI and ammonia on the anaerobic digestion of food waste.

Enhancing propionic acid production in the acidogenic fermentation of food waste facilitated by a fungal mash under neutral pH.

Fungal mash derived from Aspergillus spp. is a green enzymatic additive for food waste (FW) valorization. In this study, the production of volatile fatty acids (VFAs) and the proportion of propionic acid (PA) in VFAs were increased by utilizing a complex enzyme (CE) obtained from Aspergillus oryzae. Results showed that CE addition significantly promoted SCOD concentration in the hydrolysis at a wide pH range from 4 to 9. In contrast, the production of VFAs was influenced by pH, and the highest yields of VFAs and PA were found at pH 7. At the CE dosage of 0.2 g/g VSS, the concentration of VFAs in the FW fermentation liquid reached 38.1 g COD/L with the PA proportion up to 42.7%, which increased by 107.9% and 63.7%, respectively, relative to that in the zero-dosage group. With CE continuing to be added, the C/N ratio declined, and the primary metabolic pathway was converted from acetic acid-type to PA-type. Further investigation of the dominant microbial communities and their metabolic capacities showed that the acrylate-mediated pathway was the potential metabolic reaction in PA-type fermentation. These results indicated that CE pretreatment was a feasible strategy to enhance the PA-rich fermentation of FW under neutral pH conditions.

Efficient and Easily Recyclable Catalyst for the Alkylation Reaction of Phenol and tert-Butyl Alcohol.

tert-Butylphenol is widely used as an intermediate in organic synthesis, and the catalyst for the alkylation reaction of phenol and tert-butyl alcohol is the key to synthesizing tert-butylphenol. In our work, a catalyst, 1H-imidazole-1-acetic acid tosilate ([HIMA]OTs), was synthesized and characterized, and an efficient and easily recyclable catalytic system of an ionic liquid was established. In addition, the kinetic and thermodynamic parameters were calculated; the positive value of ΔH* indicated the endothermic nature of the alkylation reaction, and the positive value of ΔS* and negative value of ΔG* implied that the process of alkylation of phenol and tert-butyl alcohol was spontaneous in the current reaction system. The recovery experiments of [HIMA]OTs were performed, and an excellent recycling performance was obtained. This method provides a potential way for the industrial synthesis of tert-butylphenol.

Fungal mash enzymatic pretreatment combined with pH adjusting approach facilitates volatile fatty acids yield via a short-term anaerobic fermentation of food waste.

As an alternative for commercial enzyme, crude enzyme of fungal mash could promote food waste (FW) hydrolysis, but its specific effects coupled pH adjusting on the production of volatile fatty acids (VFAs) remains unknown. The crude enzyme produced from an Aspergillus awamori, named complex-amylase (CA), was added to short-term anaerobic system of FW fermentation. Results showed that adding CA significantly improved the solubility and degradability of biodegradable and non-biodegradable organics in FW, where the SCOD concentration with adding CA increased by 116.9% relative to the control but a marginal enhancement on VFAs yield. In contrast, adding CA combined with adjusting pH 8 markedly increased the VFAs production to 32.0 g COD/L, almost 10 times as much as the control. Besides, pH adjusting altered the metabolic pathway from lactate-type to butyrate-type. Adding CA coupled pH adjusting significant increase the component of butyrate compared with pH adjusting alone. Moreover, microbial community analysis indicated that adding CA reinforced proportion of the butyrate-producing bacteria (e.g., Dialister) under basic conditions, thus enhancing the butyrate metabolic pathways. This study demonstrated that fungal mash pretreatment coupled pH conditioning could be an economical way to enhance VFAs yield for FW valorization during anaerobic fermentation.

Food waste hydrolysate as a carbon source to improve nitrogen removal performance of high ammonium and high salt wastewater in a sequencing batch reactor.

The high ammonium and high salt (HAHS) wastewater generated from the anaerobic digestate of food waste is usually difficult to be treated by biological process because of its low C/N ratio. Herein, food waste hydrolysate (FWH) is rich in readily biodegradable organic matter, was utilized as carbon source to enhance the nitrogen removal of HAHS in the activated-sludge system. Results showed that compared with the control average total nitrogen removal efficiency increased from 73.4% to 94.9% and effluent declined from 281.4 mg/L to 53.9 mg/L by adding FWH at the C/N ratio of 6, satisfying the sewage discharge standard regulated by China. Besides, FWH utilization led to higher selectivity of the species responsible for nitrogen removal in related to glucose-adding group, which were dominated by Flavobacteriaceae, Melioribacteraceae, PHOS-HE36, and Rhodobacteraceae after a long-term operation. In general, FWH is an alternative carbon source to enhance nitrogen removal in HAHS wastewater treatment.

Consolidation of hydrogenotrophic methanogenesis by sulfidated nanoscale zero-valent iron in the anaerobic digestion of food waste upon ammonia stress.

The feasibility of adding sulfidated nanoscale zero-valent iron (S-nZVI) into anaerobic systems to improve anaerobic digestion of food waste (FW) under ammonia stress was evaluated in this study. The addition of S-nZVI improved the methane production compared to nanoscale zero-valent iron (nZVI), indicating that sulfidation significantly reinforced the enhancement effect of nZVI in consolidating the hydrogenotrophic methanogenesis. The promoted methanogenic performance was associated with chemical reaction and variances of microbial community induced by S-nZVI. With the characteristics of generation of Fe2+ and slow-release of H2, S-nZVI made the anaerobic system respond positively in facilitating extracellular polymeric substances secretion and optimizing the microbial community structure. Moreover, microbial community analysis showed that S-nZVI addition enriched the species related to biohydrogen production (e.g., Prevotella) and ammonia-tolerant hydrogenotrophic methanogenesis (e.g., Methanoculleus), possibly enhancing the hydrogenotrophic methanogenesis pathway to accelerate methane production. Therefore, adding S-nZVI into the anaerobic systems might propose a feasible engineering strategy to improve the methanogenic performance of the anaerobic digestion of FW upon ammonia stress.

A collaborative strategy for enhanced anaerobic co-digestion of food waste and waste activated sludge by using zero valent iron and ferrous sulfide.

The application of sulfidated zero-valent iron as an alternative used in coupled anaerobic systems to improve methane production is usually restricted by its high production costs and toxic gasses and wastewater generation. In this study, a collaborative strategy for coupling zero-valent iron (ZVI) and ferrous sulfide (FeS) together into anaerobic systems was used to evaluate the enhancement of methanogenesis during the co-digestion of food waste and waste activated sludge, with the microbial evolution and metabolic pathway revealed. Results showed that the enhanced hydrolysis and acidogenesis process of co-digestion in this coupled anaerobic system could be attributed to synergistic interactions among ZVI, FeS, and microorganisms. Furthermore, both acetoclastic and hydrogenotrophic pathways could be promoted by coupling ZVI and FeS. This study demonstrated that coupling ZVI and FeS together into anaerobic systems would be a promising method for improving the methanogenic performance for municipal solid waste treatment.

Effects of chrysophanol on hippocampal damage and mitochondrial autophagy in mice with cerebral ischemia reperfusion.

OBJECTIVE: The cerebral ischemia-reperfusion (I/R) model is crucial for the study of cerebral stroke. Chrysophanol (Chry) can protect nerve damage of mice in cerebral ischemia-reperfusion injury. This study aimed at investigating the neuroprotective effects of chrysophanol through mitochondrial autophagy in mice with ischemia-reperfusion injury. MATERIALS AND METHODS: Adult mice were stochastically divided into five groups: sham, I/R (solvent), I/R+Chry (dose, 10.0ml/kg), I/R+Chry (dose, 1.0ml/kg), and I/R+Chry (dose, 0.1ml/kg). The cerebral ischemia-reperfusion model was made in I/R and I/R+Chry groups. The changes in hippocampal formation were observed by hematoxylin and eosin (H&E) staining. The expressions of LC3B-II and LC3B-I protein in hippocampus were demonstrated by western blot (WB). The fluorescence intensities of NIX, LC3B, and mitochondria were detected by immunohistochemistry fluorescent (IF). RESULTS: Comparing with the I/R group, the I/R+Chry groups showed improvements in reducing the damage on the hippocampus, indicated by the reduced ratio of LC3B-II and LC3B-I protein, decreased fluorescence intensity of NIX and LC3B, and increased intensity of mitochondrial fluorescence. CONCLUSION: Our study showed that chrysophanol may regulate mitochondrial autophagy through NIX protein and alleviate the damage of hippocampus through decreasing the level of mitochondrial autophagy.

Rapid initiation of methanogenesis in the anaerobic digestion of food waste by acclimatizing sludge with sulfidated nanoscale zerovalent iron.

Although coupling of sulfidated nanoscale zero-valent iron (S-nZVI) into anaerobic digestion of food waste (FW) for improving methanogenesis has been reported, the specific role of S-nZVI during start-up process and its influence on subsequent methanogenesis and system stability remains unknown. In this study, S-nZVI was added into the unacclimatized sludge system to investigate its influence on microbial acclimatization and methanogenic performance. During acclimatization phase, CH4 production improved and VFAs transformation facilitated with the addition of S-nZVI. Furthermore, enzymatic activity analysis and electrochemical measurements presented direct evidence that electron transfer capacity of acclimatized sludge was significantly improved. S-nZVI favored the transition of microbial community to a robust and specialized population. During evaluation phase, acclimatized sludge still exhibited strong methanogenic ability, but the microbial community inevitably changed under the stress of FW. This research provides a novel perspective on initiating anaerobic digestion of FW for shorter start-up time and stronger methanogenesis.

Optimization ofS/Fe ratio for enhanced nitrobenzene biological removal in anaerobicSystem amended withSulfide-modified nanoscale zerovalent iron.

Anaerobic reduction of nitrobenzene (NB) can be efficiently enhanced bySupplementing withSulfide-modified nanoscale zerovalent iron (S-nZVI). In thisStudy,S/Fe ratio ofS-nZVI was further optimized for enhancing biological NB removal in anaerobicSystem amended withS-nZVI and inoculated by anaerobicSludge. The results indicated that the performance andStability of the coupled anaerobicSystem for NB reduction and aniline formation were remarkably improved byS-nZVI atS/Fe molar ratio of 0.3 (0.3S-nZVI). TheSecretion of extracellular polymericSubstances (EPS), transformation of volatile fatty acids (VFAs), yield of methane and activity ofSeveral key enzymes could be efficiently improved by 0.3S-nZVI. Furthermore,Species related to NB reduction, fermentation, electroactivity and methanogenesis could be enriched in 0.3S-nZVI coupled anaerobicSystem, with remarkable improvement in the biodiversity observed. ThisStudy demonstrated thatSulfidation would be a promising method to improve the performance of nZVI in coupled anaerobicSystems for the removal of recalcitrant nitroaromatic compounds from wastewater.

Enhanced nitrobenzene reduction by modified biochar supported sulfidated nano zerovalent iron: Comparison of surface modification methods.

In our previous study, biochar (BC) supported sulfidated nano zerovalent iron (S-nZVI@BC) was prepared for nitrobenzene (NB) reduction. In this study, in order to further improve the reduction performance of S-nZVI@BC, BC was modified before the loading of S-nZVI through three methods: oxidant (H2O2) pretreatment, alkali (NaOH) pretreatment and acid (HCl) pretreatment. The results indicated that S-nZVI could be evenly distributed onto HCl-BC due to increased surface area, negative surface charge and increased acidic functional groups on HCl-BC. At an initial concentration of 200 mg L-1, NB could be completely removed by S-nZVI@HCl-BC within a reaction time as short as 60 min, indicating rather excellent performance of S-nZVI@HCl-BC. NB reduction performance followed the order: S-nZVI@HCl-BC > S-nZVI@NaOH-BC > S-nZVI@BC > S-nZVI@H2O2-BC. The mass ratio of S-nZVI and HCl-BC was optimized in terms of NB removal efficiency, with 3:1 being identified as the best mass ratio. Furthermore, the mechanism involved in the enhanced NB reduction by S-nZVI@HCl-BC was proposed. This study demonstrated that S-nZVI@HCl-BC is a promising alternative for efficient NB removal from wastewater.

Substantially enhanced anaerobic reduction of nitrobenzene by biochar stabilized sulfide-modified nanoscale zero-valent iron: Process and mechanisms.

Nanoscale zero-valent iron (nZVI), although being increasingly used in anaerobic systems for strengthening the removal of various refractory pollutants, is limited by various inherent drawbacks, such as easy precipitation, passivation, poor mass and electron transfer. To address the above issues, biochar stabilized sulfide-modified nZVI (S-nZVI@BC) was added into an up-flow anaerobic sludge blanket (UASB) to investigate the enhancement of anaerobic biodegradation of nitrobenzene (NB) and its impacts on microbial community structure. The results demonstrated that both NB reduction and aniline formation could be substantially facilitated in S-nZVI@BC coupled system compared to other anaerobic ones coupled with nZVI or S-nZVI. The dosage of S-nZVI@BC resulted in the formation of densely packed aggregates, evidently increased the extracellular polymeric substances content, promoted the volatile fatty acids transformation and stimulated the methane yield. Furthermore, species related to fermentation (Bacteroides and Longilinea), methanogenesis (Methanosarcina and Methanomethylovorans), electroactivity (Pelobacter, Thiobacillus and Phaselicystis) as well as reduction (Desulfovibrio) were considerably enriched in S-nZVI@BC coupled system. The activities of electron transport, total adenosine triphosphate, nitroreductase and NAD(P)H, which were closely related to microbial activity and NB transformation, were increased noticeably in S-nZVI@BC coupled anaerobic system. This study demonstrated the promising potential for long-term operation and full-scale application of S-nZVI@BC coupled system for the treatment of NB containing wastewater.

A Component Decomposition Model for 3D Laser Scanning Pavement Data Based on High-Pass Filtering and Sparse Analysis.

High-precision 3D laser scanning pavement data contains rich pavement scene information and certain components associations. Moreover, for pavement maintenance and management, there is an urgent need to develop automatic methods that can extract comprehensive information about different pavement indicators simultaneously. By analyzing the frequency and sparse characteristics of pavement distresses and performance indicators-including the cracks, road markings, rutting, potholes, textures-this paper proposes 3D pavement components decomposition model (3D-PCDM) which decomposes the 3D pavement profiles into sparse components x, low-frequency components f, and vibration components t. Designed high-pass filter was first employed to separate f, then, x and t are separated by total variation de-noising which based on sparse characteristics. Decomposed x can be used to characterize the location and depth information of sparse and sparse derived signals such as cracks, road marks, grooves, and potholes in profiles. Decomposed f can be used to determine the slow deformation of pavement. While decomposed t reflects the fluctuation of the pavement material particles. Experiments were conducted using actual pavement 3D data, the decomposed components can obtain by 3D-PCDM. The effectiveness and accuracy of the x are verified by actual cracks and road markings, the accuracy of extracted sparse components is over 92.75%.

Enhanced anoxic biodegradation of pyridine coupled to nitrification in an inner loop anoxic/oxic-dynamic membrane bioreactor (A/O-DMBR).

Enhanced biodegradation of high-strength pyridine was successfully achieved in the inner loop anoxic/oxic-dynamic membrane bioreactor (A/O-DMBR) in this study. Due to the key role of dynamic membrane in biomass retention, NH4+ released from pyridine biodegradation could be effectively nitrified to NO3- in oxic zone, which was then recirculated into the anoxic zone to serve as electron acceptor for pyridine biodegradation. Acetate dosage adversely affected pyridine biodegradation, due to the competitive effect of acetate towards NO3-. Increase of recirculation ratio positively affected pyridine biodegradation, due to high availability of NO3- at high recirculation ratio. At influent pyridine concentration as high as 1500 mg L-1, effluent turbidity was well maintained below 10 NTU, indicating excellent biomass retention performance of the dynamic membrane. Microbial community analysis confirmed the enrichment of specific functional species in both anoxic and oxic zones. Stable performance during 260 days' operation confirmed the potential of A/O-DMBR for full-scale application.